Tg(Msx2-rtTA)888Lma
Transgene Detail

Summary

• Symbol: Tg(Msx2-rtTA)888Lma
• Name: transgene insertion 888, Liang Ma
• MGI ID: MGI:5546504
• Transgene: Tg(Msx2-rtTA)888Lma Location: unknown
• Alliance: Tg(Msx2-rtTA)888Lma page

Shortened and curly vibrissae, wavy body hairs, shortened forelimbs and interdigital webs in Tg(Msx2-rtTA)888Lma/? mice

Show the 1 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: Not Specified

Transgene description

Transgene Type: Transgenic (Transactivator) Mutation: Insertion Mutation details: This transgene consists of a 187 kb BAC clone, RP24-512K19, containing the endogenous Msx2 locus and flanking sequences (134 kb upstream and 47 kb downstream of Msx2; accession number AC154808) and a modified rtTA gene that replaced part of the first exon right after the start codon, followed by a FRT-flanked neomycin cassette and polyA sequences and was made using a two-step recombineering strategy. Two founding lines were established, 885 and 888. Western blot analysis failed to detect any truncated or fusion Msx2 protein. This BAC clone contains six additional predicted genes, which may be overexpressed. (J:148502)

Generation of the Tg(Msx2-rtTA)888Lma and Tg(Msx2-rtTA)885Lma transgenes

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:148502 Lin C, et al., Construction and characterization of a doxycycline-inducible transgenic system in Msx2 expressing cells. Genesis. 2009 Apr 15;47(5):352-359
• All: 2 reference(s)