Nedd4l^tm1Shkr
Targeted Allele Detail

Summary

• Symbol: Nedd4l^tm1Shkr
• Name: neural precursor cell expressed, developmentally down-regulated gene 4-like; targeted mutation 1, Sharad Kumar
• MGI ID: MGI:5546607
• Gene: Nedd4l Location: Chr18:65020776-65350899 bp, + strand Genetic Position: Chr18, 37.57 cM, cytoband E1
• Alliance: Nedd4l^tm1Shkr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205657
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A stop codon was inserted into exon 15 with a floxed neomycin resistance cassette inserted between exon 15 and 16. Cre-mediated recombination removed the neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in E18.5 lungs. (J:205657)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nedd4l Mutation: 73 strains or lines available

References

• Original: J:205657 Boase NA, et al., Respiratory distress and perinatal lethality in Nedd4-2-deficient mice. Nat Commun. 2011;2:287
• All: 6 reference(s)