Tg(Cga-icre)961Sac
Transgene Detail

Summary

• Symbol: Tg(Cga-icre)961Sac
• Name: transgene insertion 961, Sally A Camper
• MGI ID: MGI:5547616
• Synonyms: alphaGSU-iCre
• Transgene: Tg(Cga-icre)961Sac Location: unknown
• Alliance: Tg(Cga-icre)961Sac page

Transgene origin

• Strain of Origin: C57BL/6J x (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Cga-icre)961Sac expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cga (MGI:88390)

• Mutation details: A BAC (RP24-329P6) containing the Cga (alphaGSU) gene was modified by inserting a cassette composed of the sequence encoding a mammalian codon usage optimized cre recombinase (icre) and an Frt-flanked kanamycin selection marker, replacing the start codon in exon 2 of Cga with the ATG initiation codon of cre. Flpe excision removed the kanamycin selection marker. The modified BAC was injected into pronuclei of fertilized oocytes to generate transgenic mice. Line 961 was established. (J:204533)

Generation of Tg(Cga-icre)961Sac.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cga (mouse) Summary of all recombinase alleles driven by Cga. Comparative matrix view of recombinase activities Alliance mouse Cga gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:204533 Perez-Millan MI, et al., Efficient, specific, developmentally appropriate cre-mediated recombination in anterior pituitary gonadotropes and thyrotropes. Genesis. 2013 Nov;51(11):785-92
• All: 3 reference(s)