Pkm^tm1.1Mgvh
Targeted Allele Detail

Summary

• Symbol: Pkm^tm1.1Mgvh
• Name: pyruvate kinase, muscle; targeted mutation 1.1, Matthew G Vander Heiden
• MGI ID: MGI:5547750
• Synonyms: Pkm2^fl
• Gene: Pkm Location: Chr9:59563859-59586655 bp, + strand Genetic Position: Chr9, 32.03 cM
• Alliance: Pkm^tm1.1Mgvh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205213
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Modified isoform(s), No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to contain an FRT-flanked neomycin cassette upstream of exon 9, and loxP sites that flank exon 10 which is alternatively spliced only in the Pkm2 isoform. Mutant mice were bred with mice expressing FlpO recombinase (Gt(ROSA)26Sor) to remove the neomycin selection cassette. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 10 deleted in the cre-expressing tissues: pyruvate kinase M2 isoform expression is eliminated, while pyruvate kinase M1 isoform expression is unchanged. (J:205213)

Expression

In Mice Carrying this Mutation:

15 assay results

Tumor Data

List all tumor models in MMHCdb carrying Pkm^tm1.1Mgvh

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pkm Mutation: 80 strains or lines available

References

• Original: J:205213 Israelsen WJ, et al., PKM2 isoform-specific deletion reveals a differential requirement for pyruvate kinase in tumor cells. Cell. 2013 Oct 10;155(2):397-409
• All: 47 reference(s)