Gt(ROSA)26Sor^{tm9(EGFP/Rpl10a)Amc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm9(EGFP/Rpl10a)Amc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 9, Andrew P McMahon
• MGI ID: MGI:5548082
• Synonyms: EGFP-L10a, L10a, L10A-EGFP, R26^Trap
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm9(EGFP/Rpl10a)Amc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206130
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A recombinase mediated exchange (RMCE) vector was designed to contain (from 5' to 3') a promoterless neomycin cassette followed by 3 SV40 polyadenylation sites (pA), 3xpA, and in reverse orientation a fragment containing 3xIns (400 bp core region of chicken beta-globin insulator), CAGGS (CMV-IE enhancer/chicken beta-actin/rabbit beta-globin) hybrid promoter, floxed-3xpA, and a cDNA encoding an enhanced green fluorescent protein (EGFP) fused to ribosomal protein unit L10a (Rpl10a). The loxP-flanked pA cassette is designed to block transcription of EGFP/Rpl10a. (J:206130)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

The V6.5 embryonic stem (ES) cells used in the making of the mouse were targeted with a GT(ROSA)26Sor targeting vector containing an F3- and FRT-flanked ATG-F3-Puro-pA cassette followed by a PGK promoter driving expression of FLPo recombinase.

References

• Original: J:206130 Liu J, et al., Cell-specific translational profiling in acute kidney injury. J Clin Invest. 2014 Mar 3;124(3):1242-54
• All: 50 reference(s)