Sohlh1^{tm1.1(cre/ERT2)Kliu}
Targeted Allele Detail

Summary

• Symbol: Sohlh1^{tm1.1(cre/ERT2)Kliu}
• Name: spermatogenesis and oogenesis specific basic helix-loop-helix 1; targeted mutation 1.1, Kui Liu
• MGI ID: MGI:5548125
• Synonyms: Sohlh1-CreER^T2
• Gene: Sohlh1 Location: Chr2:25733007-25737260 bp, - strand Genetic Position: Chr2, 18.21 cM
• Alliance: Sohlh1^{tm1.1(cre/ERT2)Kliu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206011
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Sohlh1^{tm1.1(cre/ERT2)Kliu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sohlh1 (MGI:2684956)

• Mutation details: A targeting vector was constructed to insert a cre/ERT2-Frt-neo-Frt cassette into the Sohlh1 gene by homologous recombination. The cre cassette was inserted at the ATG start codon, replacing exons 1-8. Chimeras were bred to C57BL/6 mice, and F1 animals were crossed to flp-expressing mice to remove the neo marker. (J:206011)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sohlh1 (mouse) Summary of all recombinase alleles driven by Sohlh1. Comparative matrix view of recombinase activities Alliance mouse Sohlh1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sohlh1 Mutation: 15 strains or lines available

References

• Original: J:206011 Zheng W, et al., Two classes of ovarian primordial follicles exhibit distinct developmental dynamics and physiological functions. Hum Mol Genet. 2014 Feb 15;23(4):920-8
• All: 2 reference(s)