Eef1a1^tm1Rck
Targeted Allele Detail

Summary

• Symbol: Eef1a1^tm1Rck
• Name: eukaryotic translation elongation factor 1 alpha 1; targeted mutation 1, Jeffrey M Friedman
• MGI ID: MGI:5548150
• Synonyms: EEF1A1-LSL.EGFPL10
• Gene: Eef1a1 Location: Chr9:78385735-78389006 bp, - strand Genetic Position: Chr9, 43.65 cM, cytoband E1
• Alliance: Eef1a1^tm1Rck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206002
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The cDNA encoding a fusion of an EGFP protein with the L10 (large subunit ribosomal protein L10) was inserted into a vector downstream of a floxed 'STOP' cassette and a neomycin selection marker and upstream of a polyadenylation signal. Homologous recombination in ES cells inserted this sequence into the first intron of Eef1a1. Cre-mediated recombination of this allele results in expression of the EGFPL10 protein. (J:206002)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Eef1a1 Mutation: 60 strains or lines available

References

• Original: J:206002 Stanley S, et al., Profiling of Glucose-Sensing Neurons Reveals that GHRH Neurons Are Activated by Hypoglycemia. Cell Metab. 2013 Oct 1;18(4):596-607
• All: 18 reference(s)