Pdgfa^tm2Cbet
Targeted Allele Detail

Summary

• Symbol: Pdgfa^tm2Cbet
• Name: platelet derived growth factor, alpha; targeted mutation 2, Christer Betsholtz
• MGI ID: MGI:5548172
• Synonyms: Pdgfa^deltaex6
• Gene: Pdgfa Location: Chr5:138961769-138983125 bp, - strand Genetic Position: Chr5, 77.65 cM
• Alliance: Pdgfa^tm2Cbet page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205009
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph, Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette inserted into intron 5, deleting the 5' splice site upstream of exon 6. This allele is predicted to produce only the short form transcript but not express the long form. Q-PCR confirmed reduced total transcript expression in the lung and skin, but not other tissues. Authors state that this allele might be hypomorphic in the lungs but not other tissues. (J:205009)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdgfa Mutation: 20 strains or lines available

References

• Original: J:205009 Andrae J, et al., Analysis of mice lacking the heparin-binding splice isoform of platelet-derived growth factor A. Mol Cell Biol. 2013 Oct;33(20):4030-40
• All: 1 reference(s)