Synm^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Synm^{tm1.1(KOMP)Vlcg}
• Name: synemin, intermediate filament protein; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5548847
• Gene: Synm Location: Chr7:67379909-67409490 bp, - strand Genetic Position: Chr7, 37.12 cM
• Alliance: Synm^{tm1.1(KOMP)Vlcg} page
• IMPC: Synm gene page

Mutation origin

• Mutant Cell Line: 14923A-H9
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: Cre-mediated excision of the parental Synm^{tm1(KOMP)Vlcg} allele resulted in the removal of the neomycin selection cassette and exons 2 and 3, leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
  RT-PCR analysis of mRNA from skeletal muscle of homozygous mutant and control mice showed that the transcript is absent from the homozygotes but present in controls. Western blot analysis of protein supernatants prepared from extracts of tibialis anterior muscles confirmed the absence of encoded protein in homozygous mutant muscles. (J:204739, J:224030)

Expression

In Mice Carrying this Mutation:	182 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Synm Mutation: 58 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 8 reference(s)