Cd4^{tm1(cre/ERT2)Thbu}
Targeted Allele Detail

Summary

• Symbol: Cd4^{tm1(cre/ERT2)Thbu}
• Name: CD4 antigen; targeted mutation 1, Thorsten Buch
• MGI ID: MGI:5549971
• Synonyms: CD4-CreER^t2
• Gene: Cd4 Location: Chr6:124841655-124865184 bp, - strand Genetic Position: Chr6, 59.17 cM
• Alliance: Cd4^{tm1(cre/ERT2)Thbu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204579
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Cd4^{tm1(cre/ERT2)Thbu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cd4 (MGI:88335)

• Mutation details: A targeting vector was designed to replace part of exon 2 including the ATG codon of the Cd4 locus with the cre/ERT2 fusion gene and Frt-flanked neo marker. Homologous recombination in ES cells generated the knock-in allele. (J:204579)

Generation of Cd4^{tm1(cre/ERT2)Thbu}.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cd4 (mouse) Summary of all recombinase alleles driven by Cd4. Alliance mouse Cd4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd4 Mutation: 85 strains or lines available

References

• Original: J:204579 Sledzinska A, et al., TGF-beta signalling is required for CD4(+) T cell homeostasis but dispensable for regulatory T cell function. PLoS Biol. 2013 Oct;11(10):e1001674
• All: 22 reference(s)