Mycl^tm1.1Kmm
Targeted Allele Detail

Summary

• Symbol: Mycl^tm1.1Kmm
• Name: v-myc avian myelocytomatosis viral oncogene lung carcinoma derived; targeted mutation 1.1, Kenneth Murphy
• MGI ID: MGI:5550274
• Synonyms: Mycl1^gfp
• Gene: Mycl Location: Chr4:122889445-122896278 bp, + strand Genetic Position: Chr4, 57.36 cM
• Alliance: Mycl^tm1.1Kmm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206430
• Parent Cell Line: EDJ22 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector was designed to replace the first coding exon with a green fluorescent protein (GFP) sequence and a loxP-flanked neomycin resistance (neo) cassette. Mutant mice were bred with Tg(CMV-cre)1Cgn mice (on a C57BL/6 congenic background) to remove the neo selection cassette. qPCR failed to detect mRNA in mutants. GFP expression is detected in dendritic cells in both lymphoid and non-lymphoid peripheral tissues. (J:206430)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mycl Mutation: 19 strains or lines available

References

• Original: J:206430 KC W, et al., L-Myc expression by dendritic cells is required for optimal T-cell priming. Nature. 2014 Mar 13;507(7491):243-7
• All: 4 reference(s)