Samhd1^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Samhd1^{tm1d(EUCOMM)Wtsi}
• Name: SAM domain and HD domain, 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5551758
• Synonyms: SAMHD1^delta
• Gene: Samhd1 Location: Chr2:156939454-156977016 bp, - strand Genetic Position: Chr2, 78.0 cM, cytoband H2
• Alliance: Samhd1^{tm1d(EUCOMM)Wtsi} page
• IMPC: Samhd1 gene page

Mutation origin

• Mutant Cell Line: EPD0424_3_A06
• Germline Transmission: Earliest citation of germline transmission: J:202757
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 156971510 of Chromosome 2 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 156972268. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the trapping cassette. Cre-mediated recombination removed exon 2. Western blot analysis confirmed the absence of protein expression in splenocytes. (J:202757)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Samhd1 Mutation: 37 strains or lines available

References

• Original: J:202757 Behrendt R, et al., Mouse SAMHD1 has antiretroviral activity and suppresses a spontaneous cell-intrinsic antiviral response. Cell Rep. 2013 Aug 29;4(4):689-96
• All: 6 reference(s)