Washc1^tm1.1Xya
Targeted Allele Detail

Summary

• Symbol: Washc1^tm1.1Xya
• Name: WASH complex subunit 1; targeted mutation 1.1, Xiao Yang
• MGI ID: MGI:5551785
• Gene: Washc1 Location: Chr17:66418540-66427498 bp, + strand Genetic Position: Chr17, 35.26 cM, cytoband F3-F4
• Alliance: Washc1^tm1.1Xya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:202483
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 3. A neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 3. Cre-mediated recombination removed exon 3 and the resistance cassette. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:202483)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Washc1 Mutation: 13 strains or lines available

References

• Original: J:202483 Xia P, et al., WASH inhibits autophagy through suppression of Beclin 1 ubiquitination. EMBO J. 2013 Oct 16;32(20):2685-96
• All: 2 reference(s)