Snord50a/Snord50b^tm1.1Soen
Targeted Allele Detail

Summary

• Symbol: Snord50a/Snord50b^tm1.1Soen
• Name: small nucleolar RNA, C/D box 50A; targeted mutation 1.1, Yuuichi Soeno
• MGI ID: MGI:5552747
• Synonyms: deltamU50HG-b
• Gene: Snord50a Location: Chr9:88403582-88403653 bp, - strand Genetic Position: Chr9, Syntenic
• Alliance: Snord50a/Snord50b^tm1.1Soen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206419
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: The first snoRNA 50 (mU50) sequence was replaced with a 68 bp multiple cloning site from pBluescriptII KSII plasmid containing an EcoRV site. The second mU50 site was replaced with a floxed neomycin resistance cassette. Cre-mediated recombination removed the resistance cassette. Northern blot analysis confirmed the absence of expression in the spleen. (J:206419)

Generation of the Snord50a/Snord50b^tm1.1Soen allele

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Snord50a Mutation: 0 strains or lines available

References

• Original: J:206419 Soeno Y, et al., Generation of a mouse model with down-regulated U50 snoRNA (SNORD50) expression and its organ-specific phenotypic modulation. PLoS One. 2013;8(8):e72105
• All: 1 reference(s)