Nle1^tm1.1Cota
Targeted Allele Detail

Summary

• Symbol: Nle1^tm1.1Cota
• Name: notchless homolog 1; targeted mutation 1.1, Michel Cohen-Tannoudji
• MGI ID: MGI:5553357
• Synonyms: Nle1^flox
• Gene: Nle1 Location: Chr11:82791594-82799237 bp, - strand Genetic Position: Chr11, 50.3 cM
• Alliance: Nle1^tm1.1Cota page

Severe hypocellularity in Nle1^tm1Cba/Nle1^tm1.1Cota Gt(ROSA)26Sor^{tm2(cre/ERT2)Brn}/Gt(ROSA)26Sor⁺ bone marrow, with capillaries saturated with erythrocytes

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204063
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: A loxP site was inserted upstream of exon 4. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 9. Flp-mediated recombination removed the resistance cassette and left exons 4 through 9 floxed. (J:204063)

Generation of the Nle1^tm1.1Cota and Nle1^tm1.2Cota alleles

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nle1 Mutation: 27 strains or lines available

References

• Original: J:204063 Le Bouteiller M, et al., Notchless-dependent ribosome synthesis is required for the maintenance of adult hematopoietic stem cells. J Exp Med. 2013 Oct 21;210(11):2351-69
• All: 4 reference(s)