Tg(JAK2*V617F)FF1Rsko
Transgene Detail
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Symbol: |
Tg(JAK2*V617F)FF1Rsko |
Name: |
transgene insertion FF1, Radek C Skoda |
MGI ID: |
MGI:5553468 |
Synonyms: |
JAK2-V617F |
Transgene: |
Tg(JAK2*V617F)FF1Rsko Location: unknown Genetic Position: Chr8, Syntenic
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Alliance: |
Tg(JAK2*V617F)FF1Rsko page
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Transgene Type: |
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Transgenic (Humanized sequence, Inserted expressed sequence) |
Mutation: |
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Insertion
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Tg(JAK2*V617F)FF1Rsko expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
JAK2 (3717) |
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Mutation details: The human BAC, CTD2025A15, containing JAK2 exons 1-12 was combined with a partial cDNA encoding JAK2 exons 13-25 containing the V617F mutation and a polyadenylation signal. To create an inducible Flip-Flop transgene, this construct was modified by inserting a lox66 site into intron 12 and a lox71 site after the polyadenylation signal and the segment between the loxP sites was inverted. Cre-recombination of antiparallel loxP sites flips the orientation of the cDNA and places the intron 12 splice acceptor into the correct position to allow proper splicing of the transgenic pre-mRNA. Recombination generates one wild-type loxP and one double mutant lox66/77 site, which is no longer a substrate for the cre recombinase. This line carries 9 copies of the transgene that integrated in a single chromosomal locus on chromosome 8, band A1.
(J:134364)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:134364 Tiedt R, et al., Ratio of mutant JAK2-V617F to wild-type Jak2 determines the MPD phenotypes in transgenic mice. Blood. 2008 Apr 15;111(8):3931-40 |
All: |
16 reference(s) |
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