Ddx4^{tm1.1(cre)Dcp}
Targeted Allele Detail

Summary

• Symbol: Ddx4^{tm1.1(cre)Dcp}
• Name: DEAD box helicase 4; targeted mutation 1.1, David C Page
• MGI ID: MGI:5554579
• Synonyms: Ddx4^{tm1.1(cre/mOrange)Dcp}, Mvh^Cre-mOrange
• Gene: Ddx4 Location: Chr13:112734867-112789009 bp, - strand Genetic Position: Chr13, 63.87 cM
• Alliance: Ddx4^{tm1.1(cre)Dcp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198777
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Ddx4^{tm1.1(cre)Dcp} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ddx4 (MGI:102670)

• Mutation details: The sequence encoding a cre-mOrange fusion protein was inserted into the Ddx4 (Mvh) locus by homologous recombination with a targeting vector in ES cells. Part of exon 1 and intron 1 were replaced by the cre-mOrange sequence. A PGK-neo selection marker was inserted in the initial targeting, and was later removed by mating with FLPe-expressing mice. The mOrange fluorescent reporter in the construct does not appear to be functional. (J:198777)

Generation of Ddx4^{tm1.1(cre)Dcp}

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ddx4 (mouse) Summary of all recombinase alleles driven by Ddx4. Comparative matrix view of recombinase activities Alliance mouse Ddx4 gene page

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddx4 Mutation: 59 strains or lines available

References

• Original: J:198777 Hu YC, et al., Tumor suppressor gene Rb is required for self-renewal of spermatogonial stem cells in mice. Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12685-90
• All: 12 reference(s)