Actr8^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Actr8^{tm1.1(KOMP)Vlcg}
• Name: ARP8 actin-related protein 8; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5554924
• Synonyms: Actr8^-
• Gene: Actr8 Location: Chr14:29700294-29717409 bp, + strand Genetic Position: Chr14, 18.37 cM
• Alliance: Actr8^{tm1.1(KOMP)Vlcg} page
• IMPC: Actr8 gene page

Actr8^{tm1.1(KOMP)Vlcg}/Actr8^{tm1.1(KOMP)Vlcg} mice exhibit embryonic lethality, with arrest prior to gastrulation and no embryos at E9.5. Embryos form a rudimentary egg-cylinder but no primitive streak of hallmarks of gastrulation are seen at E7.5.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: 19162A-A6
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 14639bp between positions 30791655-30806293 of Chromosome 14 (Genome Build37), and subsequent Cre-mediated excision deleted the neomycin selection cassette. (J:136110, J:188991)

Expression

In Mice Carrying this Mutation:	15 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Actr8 Mutation: 32 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 7 reference(s)