Sema3g^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Sema3g^{tm1a(EUCOMM)Hmgu}
• Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3G; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5555120
• Gene: Sema3g Location: Chr14:30939830-30952309 bp, + strand Genetic Position: Chr14, 19.09 cM
• IMPC: Sema3g gene page

Mutation origin

• Mutant Cell Lines: HEPD0963_5_A02, HEPD0963_5_B03, HEPD0963_5_C04, HEPD0963_5_D01, HEPD0963_5_E02, HEPD0963_5_F01, HEPD0963_5_F03, HEPD0963_5_G02
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo
• Mutation details: The Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo cassette was inserted at position 30944407 of Chromosome 14 (Build GRCm39) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 30945763. The critical artificial exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors. (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Sema3g Mutation: 19 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)