Pld1^tm1.1Gdp
Targeted Allele Detail

Summary

• Symbol: Pld1^tm1.1Gdp
• Name: phospholipase D1; targeted mutation 1.1, Gilbert Di Paolo
• MGI ID: MGI:5556064
• Synonyms: Pld1^tm2.1Mafr
• Gene: Pld1 Location: Chr3:27992844-28187511 bp, + strand Genetic Position: Chr3, 11.15 cM
• Alliance: Pld1^tm1.1Gdp page

Reduced hepatic autophagy in starved Pld1^tm1.1Gdp/Pld1^tm1.1Gdp mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207283
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6 x 129/SvJ

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 13. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 14. This allele is created by using either Cre-mediated recombination to remove exon 13, exon 14 and the selection cassette or Flp-mediated recombination to remove the FRT-flanked neo cassette, followed by Cre-mediated recombination to remove exons 13 and 14 . Western blot analysis confirmed the absence of protein expression in the liver and mouse embryonic fibroblasts. (J:207283)

Generation of the Pld1^tm1.1Gdp allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pld1 Mutation: 64 strains or lines available

References

• Original: J:207283 Dall'Armi C, et al., The phospholipase D1 pathway modulates macroautophagy. Nat Commun. 2010;1:142
• All: 17 reference(s)