Tg(hs218-Ccre,-lacZ)1Fawa
Transgene Detail

Summary

• Symbol: Tg(hs218-Ccre,-lacZ)1Fawa
• Name: transgene insertion 1, Fan Wang
• MGI ID: MGI:5556213
• Synonyms: CreC218
• Transgene: Tg(hs218-Ccre,-lacZ)1Fawa Location: unknown
• Alliance: Tg(hs218-Ccre,-lacZ)1Fawa page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(hs218-Ccre,-lacZ)1Fawa expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  human	hs218

• Mutation details: The C-terminal fragment of cre recombinase (aa 60-343) fused to the intein-C peptide fragment (36 C terminal amino acids) of the DnaE split intein sequence was cloned into a vector containing the human enhancer 218 sequence (hs218) and an Hsp68 minimal promoter and lacZ reporter. The final construct included an IRES sequence separating the lacZ gene and Ccre-intein-C. Injection of the linearized construct into pronuclei was performed to generated transgenic founders. Line numbers were not identified in the publication. (J:207270)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	hs218 (human) Summary of all recombinase alleles driven by hs218.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Examples of reporter expression driven by this and other human enhancers in mouse embryos produced by transient transgenesis can be viewed at http://enhancer.lbl.gov.

References

• Original: J:207270 Wang P, et al., Intersectional Cre driver lines generated using split-intein mediated split-Cre reconstitution. Sci Rep. 2012;2:497
• All: 1 reference(s)