Rock1^tm1.2Itl
Targeted Allele Detail

Summary

• Symbol: Rock1^tm1.2Itl
• Name: Rho-associated coiled-coil containing protein kinase 1; targeted mutation 1.2, inGenious Targeting Laboratory
• MGI ID: MGI:5556290
• Gene: Rock1 Location: Chr18:10064401-10182045 bp, - strand Genetic Position: Chr18, 4.91 cM, cytoband A2
• Alliance: Rock1^tm1.2Itl page

Absence of autophagosomes in Rock1^tm1.2Itl/Rock1^tm1.2Itl starved hearts

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207251
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: An FRT- and loxP-flanked neomycin resistance cassette was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exon 5. Western blot analysis confirmed the absence of protein expression in the heart. (J:207251)

Generation of the Rock1^tm1.1Itl and Rock1^tm1.2Itl alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rock1 Mutation: 60 strains or lines available

References

• Original: J:207251 Gurkar AU, et al., Identification of ROCK1 kinase as a critical regulator of Beclin1-mediated autophagy during metabolic stress. Nat Commun. 2013;4:2189
• All: 1 reference(s)