Col1a1^{tm1(tetO-cre)Haho}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-cre)Haho}
• Name: collagen, type I, alpha 1; targeted mutation 1, Hanno Hock
• MGI ID: MGI:5558024
• Synonyms: R26^M2rtTA;Col1a1^TetOP-Cre, R26-M2rtTA::TetOP-Cre, TetOP-Cre
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-cre)Haho} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207446
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-cre)Haho} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  bacteria	tetO

• Mutation details: A "TetOP-Cre flip-in plasmid" (containing a splice acceptor-polyA sequence and the tetracycline operator elements (tet)) fused to a CMV minimal promoter upstream of a cre recombinase cassette) was used to treat ES cells containing an FRT-PGK-neo-hygro-FRT cassette inserted into the 3' UTR of the Col1a1 locus (Col1a1^{tm13(neo/hygro)Jae}). The "flip-in" vector was coinjected with a Flpe-encoding plasmid so that flippase-mediated recombination inserted the tetO-cre sequence into the Col1a1 3' UTR. The Col1a1^{tm13(neo/hygro)Jae} ES cells also contain the Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} allele. The twice-targeted ES cells were used to generate chimeras and germline animals. By treating this mouse line with doxycycline, rtTA*M2 binds the TetOP promoter and cre is expressed. (J:207446)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	tetO (bacteria) Summary of all recombinase alleles driven by tetO.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:207446 Foudi A, et al., Distinct, strict requirements for Gfi-1b in adult bone marrow red cell and platelet generation. J Exp Med. 2014 May 5;211(5):909-27
• All: 4 reference(s)