Il5^{tm1.1(icre)Lky}
Targeted Allele Detail

Summary

• Symbol: Il5^{tm1.1(icre)Lky}
• Name: interleukin 5; targeted mutation 1.1, Richard M Locksley
• MGI ID: MGI:5558083
• Synonyms: Il5^-, Il5^R, Il5^Red5, R5
• Gene: Il5 Location: Chr11:53611621-53615930 bp, + strand Genetic Position: Chr11, 31.99 cM, cytoband A5/B1
• Alliance: Il5^{tm1.1(icre)Lky} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206097
• Parent Cell Line: PRX-B6T (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutation: Insertion
• Il5^{tm1.1(icre)Lky} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Il5 (MGI:96557)

• Mutation details: A targeting vector with a cassette composed of, (from 5' to 3'), a partial rabbit beta-globin exon 2-3 genomic sequence, the tdTomato coding sequence, an internal ribosomal entry site (IRES), a humanized cre recombinase sequence and a bovine growth hormone polyadenlyation signal was flanked by Il5 homology arms and linearized for homologous recombination in ES cells. The cassette was inserted at the transcriptional start ATG codon of the Il5 gene. Chimeric males were crossed to CMV-cre females to excise the neo marker, and then male offspring were bred to wild-type B6 females to remove the CMV-cre from the background. Mice express tdTomato and cre in cells where Il5 is expressed. (J:206097)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Il5 (mouse) Summary of all recombinase alleles driven by Il5. Alliance mouse Il5 gene page

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il5 Mutation: 31 strains or lines available

References

• Original: J:206097 Nussbaum JC, et al., Type 2 innate lymphoid cells control eosinophil homeostasis. Nature. 2013 Oct 10;502(7470):245-8
• All: 46 reference(s)