Fkbpl^{tm1.1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Fkbpl^{tm1.1(KOMP)Wtsi}
• Name: FK506 binding protein-like; targeted mutation 1.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5558157
• Synonyms: Fkbpl^-
• Gene: Fkbpl Location: Chr17:34863738-34865298 bp, + strand Genetic Position: Chr17, 18.22 cM
• Alliance: Fkbpl^{tm1.1(KOMP)Wtsi} page
• IMPC: Fkbpl gene page

Mutation origin

• Mutant Cell Line: EPD0466_1_C01
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: Cre-mediated excision of the parental Fkbpl^{tm1(KOMP)Wtsi} allele resulted in the removal of the neomycin selection cassette and exon 2 leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Real-time quantitative PCR analysis revealed a ~2-fold reduction of mRNA expression in spleen tissue lysates from heterozygous mutant mice; however, serum protein levels were downregulated up to 4-fold relative to wild-type controls. (J:204739, J:226964)

Expression

In Mice Carrying this Mutation:

36 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fkbpl Mutation: 41 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 4 reference(s)