Slx1b^tm1.1Jrou
Targeted Allele Detail

Summary

• Symbol: Slx1b^tm1.1Jrou
• Name: SLX1 structure-specific endonuclease subunit homolog B (S. cerevisiae); targeted mutation 1.1, John Rouse
• MGI ID: MGI:5559221
• Gene: Slx1b Location: Chr7:126288640-126294956 bp, - strand Genetic Position: Chr7, 69.25 cM, cytoband F4
• Alliance: Slx1b^tm1.1Jrou page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204996
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette with an IRES and a 5' loxP site was inserted upstream of the coding sequence in exon 1. An additional loxP site was inserted downstream of exon 1. Cre-mediated recombination removed the coding sequence in exon 1 and the selection cassette. Western blot analysis confirmed the absence of protein expression in testis and mouse embryonic fibroblasts. (J:204996)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slx1b Mutation: 27 strains or lines available

References

• Original: J:204996 Castor D, et al., Cooperative control of holliday junction resolution and DNA repair by the SLX1 and MUS81-EME1 nucleases. Mol Cell. 2013 Oct 24;52(2):221-33
• All: 2 reference(s)