Phc2^tm3.1Hko
Targeted Allele Detail

Summary

• Symbol: Phc2^tm3.1Hko
• Name: polyhomeotic 2; targeted mutation 3.1, Haruhiko Koseki
• MGI ID: MGI:5559319
• Synonyms: Phc2^L307R
• Gene: Phc2 Location: Chr4:128548495-128646674 bp, + strand Genetic Position: Chr4, 62.12 cM
• Alliance: Phc2^tm3.1Hko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204987
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Single point mutation
• Mutation details: The final coding exon was replaced with one in which a T to C point mutation results in the amino acid substitution of arginine for lysine at position 307 (L307R). Cre-mediated recombination removed the neomycin resistance cassette inserted downstream of the stop codon. (J:204987)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phc2 Mutation: 83 strains or lines available

References

• Original: J:204987 Isono K, et al., SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Dev Cell. 2013 Sep 30;26(6):565-77
• All: 1 reference(s)