Esr1^{tm1.1(cre)And}
Targeted Allele Detail

Summary

• Symbol: Esr1^{tm1.1(cre)And}
• Name: estrogen receptor 1 (alpha); targeted mutation 1.1, David J Anderson
• MGI ID: MGI:5559339
• Synonyms: Esr1^cre, Esr1cre
• Gene: Esr1 Location: Chr10:4561989-4955633 bp, + strand Genetic Position: Chr10, 2.03 cM
• Alliance: Esr1^{tm1.1(cre)And} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207677
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (RMCE-ready, Recombinase)
• Mutation: Insertion
• Esr1^{tm1.1(cre)And} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Esr1 (MGI:1352467)

• Mutation details: The Esr1-cre targeting vector was designed to insert an attP site, a 2A oligopeptide (mediates ribosomal skipping), the Cre recombinase gene, a frt-flanked PGKneo-polyA cassette, and an attP site within the 3' UTR of the estrogen receptor 1 alpha gene (Esr1). The targeting construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Chimeric males were bred with 129S6/SvEvTac females to establish the colony. Heterozygotes were bred to ACT-FLPe mice to remove the frt-flanked PGKneo-polyA cassette. Mice were bred to remove the ACT-FLPe transgene from the background. (J:207677)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Esr1 (mouse) Summary of all recombinase alleles driven by Esr1. Comparative matrix view of recombinase activities Alliance mouse Esr1 gene page

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Esr1 Mutation: 68 strains or lines available

References

• Original: J:207677 Lee H, et al., Scalable control of mounting and attack by Esr1+ neurons in the ventromedial hypothalamus. Nature. 2014 May 29;509(7502):627-32
• All: 35 reference(s)