Crh^{tm1.1(cre)Dolsn}
Targeted Allele Detail

Summary

• Symbol: Crh^{tm1.1(cre)Dolsn}
• Name: corticotropin releasing hormone; targeted mutation 1.1, David P Olson
• MGI ID: MGI:5559540
• Synonyms: Crf^cre, CRF-ires-Cre, Crh-IRES-Cre
• Gene: Crh Location: Chr3:19747565-19749560 bp, - strand Genetic Position: Chr3, 5.75 cM
• Alliance: Crh^{tm1.1(cre)Dolsn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207472
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Crh^{tm1.1(cre)Dolsn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Crh (MGI:88496)

• Mutation details: A selection cassette containing an internal ribosomal entry sequence (IRES) linked to cre and an frt-flanked kanamycin gene was inserted immediately downstream of the stop codon in exon 1 of the Crh gene contained in a BAC using recombineering methods. This construct was used to isolate a targeting plasmid with the cre-kanamycin cassette with upstream and downstream Crh homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals. (J:207472)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Crh (mouse) Summary of all recombinase alleles driven by Crh. Comparative matrix view of recombinase activities Alliance mouse Crh gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crh Mutation: 42 strains or lines available

References

• Original: J:207472 Krashes MJ, et al., An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger. Nature. 2014 Mar 13;507(7491):238-42
• All: 16 reference(s)