Tg(CAG-dsRed2/RNAi:Tardbp)6Zxu
Transgene Detail

Summary

• Symbol: Tg(CAG-dsRed2/RNAi:Tardbp)6Zxu
• Name: transgene insertion 6, Zuoshang Xu
• MGI ID: MGI:5559582
• Synonyms: amiR-TDP43i
• Transgene: Tg(CAG-dsRed2/RNAi:Tardbp)6Zxu Location: unknown
• Alliance: Tg(CAG-dsRed2/RNAi:Tardbp)6Zxu page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Knockdown, Reporter)
• Mutation: Insertion
• Tg(CAG-dsRed2/RNAi:Tardbp)6Zxu involves 1 genes/genome features (Tardbp) View all
• Mutation details: The transgenic construct contains (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a loxP site, an enhanced green fluorescent protein sequence followed by three polyadenylation signals, a second loxP site, a dsRed2 fluorescent protein sequence, a synthetic artificial intron containing the amiR-TDP43 precursor miRNA sequence, and a polyA signal. The synthetic artificial intron utilized here was designed to contain typical intron splicing regulatory elements and was specifically placed 10 nucleotides downstream of the RFP stop codon because introns within 50 nucleotides downstream from the stop codon are found in some eukaryotic mRNAs and do not cause non-sense mediated decay (NMD). The amiR-TDP43 sequence (miR-TDP-43b; 5'-ATGCTGAACCTAAGCATAA-3') is an artificial precursor miRNA sequence encoding a short hairpin RNA specifically synthesized to target the RNA of mouse TAR DNA binding protein (TBP-43 or Tardbp). (J:207750)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:207750 Yang C, et al., Partial loss of TDP-43 function causes phenotypes of amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):E1121-9
• All: 1 reference(s)