Calca^{tm1.1(cre/EGFP)Rpa}
Targeted Allele Detail

Summary

• Symbol: Calca^{tm1.1(cre/EGFP)Rpa}
• Name: calcitonin/calcitonin-related polypeptide, alpha; targeted mutation 1.1, Richard D Palmiter
• MGI ID: MGI:5559692
• Synonyms: Calca^Cre, CGRP^GFP
• Gene: Calca Location: Chr7:114230713-114235592 bp, - strand Genetic Position: Chr7, 59.99 cM
• Alliance: Calca^{tm1.1(cre/EGFP)Rpa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206070
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Calca^{tm1.1(cre/EGFP)Rpa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Calca (MGI:2151253)

• Mutation details: The targeting vector used for homologous recombination contained a cre/GFP fusion sequence (cre fused in frame with EGFP at the C-terminus), N-terminal nuclear localization signal and myc tag, and frt-flanked PGK-neo marker. Cre was inserted at the initiation ATG codon in exon 2 of the gene. Chimeric animals were bred with Flper mice to remove the PGK-neo selection marker. (J:206070)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Calca (mouse) Summary of all recombinase alleles driven by Calca. Comparative matrix view of recombinase activities Alliance mouse Calca gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Calca Mutation: 23 strains or lines available

References

• Original: J:206070 Carter ME, et al., Genetic identification of a neural circuit that suppresses appetite. Nature. 2013 Nov 7;503(7474):111-4
• All: 28 reference(s)