Cdk5r1^tm2.1Lht
Targeted Allele Detail

Summary

• Symbol: Cdk5r1^tm2.1Lht
• Name: cyclin dependent kinase 5, regulatory subunit 1; targeted mutation 2.1, Li-Huei Tsai
• MGI ID: MGI:5560495
• Synonyms: delta p35-3xHA, deltap35KI
• Gene: Cdk5r1 Location: Chr11:80367849-80372010 bp, + strand Genetic Position: Chr11, 47.94 cM
• Alliance: Cdk5r1^tm2.1Lht page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208030
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted
• Mutations: Intragenic deletion, Nucleotide substitutions
• Mutation details: The deltap35-3xHA knock-in cassette encodes for deletion of the six amino acid residues adjacent to the calpain-cleavage site (delta93-98) and replaces the alanine residue in the cleavage site for leucine., The cassette was generated via two sequential recombination reactions. The final targeting vector contains this deltap35-3xHA knock-in cassette and a loxP site-flanked NEO cassette. Mutant mice were bred with Meox2-cre mice to remove the neo selection cassette. (J:208030)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdk5r1 Mutation: 13 strains or lines available

References

• Original: J:208030 Seo J, et al., Activity-Dependent p25 Generation Regulates Synaptic Plasticity and Abeta-Induced Cognitive Impairment. Cell. 2014 Apr 10;157(2):486-98
• All: 4 reference(s)