Hcn4^tm1Nnka
Targeted Allele Detail

Summary

• Symbol: Hcn4^tm1Nnka
• Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4; targeted mutation 1, Noriyuki Nakashima
• MGI ID: MGI:5560747
• Synonyms: HCN4-Tet, Hcn4^tm1(tTA), HCN4^tTA_TRE
• Gene: Hcn4 Location: Chr9:58730695-58770458 bp, + strand Genetic Position: Chr9, 31.8 cM, cytoband C
• Alliance: Hcn4^tm1Nnka page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207953
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The targeting construct introduced upstream of the translation start site a tetracycline transactivator, floxed neomycin resistance cassette, polyadenylation sequence and tetracycline response element with CMV promoter (teto5-CMV). In the absence of doxycycline, the endogenous gene is over-expressed. Doxycycline treatment reduces expression. (J:207953)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hcn4 Mutation: 67 strains or lines available

References

• Original: J:207953 Nakashima N, et al., Hyperpolarisation-activated cyclic nucleotide-gated channels regulate the spontaneous firing rate of olfactory receptor neurons and affect glomerular formation in mice. J Physiol. 2013 Apr 1;591(Pt 7):1749-69
• All: 2 reference(s)