Trh^{tm1.1(cre)Mjkr}
Targeted Allele Detail

Summary

• Symbol: Trh^{tm1.1(cre)Mjkr}
• Name: thyrotropin releasing hormone; targeted mutation 1.1, Michael J Krashes
• MGI ID: MGI:5562874
• Synonyms: Trh-IRES-Cre
• Gene: Trh Location: Chr6:92219042-92221631 bp, - strand Genetic Position: Chr6, 41.03 cM
• Alliance: Trh^{tm1.1(cre)Mjkr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207472
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Trh^{tm1.1(cre)Mjkr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Trh (MGI:98823)

• Mutation details: Using recombineering methods, a selection cassette containing an internal ribosomal entry sequence (IRES) linked to cre and an frt-flanked kanamycin gene was inserted immediately downstream of the stop codon in exon 2 of the Trh gene in a BAC. This construct was used to isolate a targeting plasmid with the cre-kanamycin cassette with upstream and downstream Trh homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals, however, a partial neo (164 bp fragment of the 3' end of neo) remains between the IRES-Cre and polyA sequences. (J:101977, J:207472)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Trh (mouse) Summary of all recombinase alleles driven by Trh. Comparative matrix view of recombinase activities Alliance mouse Trh gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trh Mutation: 23 strains or lines available

References

• Original: J:207472 Krashes MJ, et al., An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger. Nature. 2014 Mar 13;507(7491):238-42
• All: 8 reference(s)