Del(8Defb1-Defb13)1Jrdo
Targeted Allele Detail

Summary

• Symbol: Del(8Defb1-Defb13)1Jrdo
• Name: deletion, Chr 8, Julia R Dorin 1
• MGI ID: MGI:5562924
• Synonyms: Defbdelta9
• Gene: Del(8Defb1-Defb13)1Jrdo Location: unknown Genetic Position: Chr8, Syntenic

Increased number of detached heads in the cauda epididymal sperm from Del(8Defb1-Defb13)1Jrdo/Del(8Defb1-Defb13)1Jrdo mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204187
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intergenic deletion, Intragenic deletion Del(8Defb1-Defb13)1Jrdo involves 15 genes/genome features (Pnhdlc3, Scc8, Eae36 ...) View all Mutation details: A MICER clone carrying exons 1 and 2 of the HPRT gene, a neo cassette, a loxP site and Tyrosinase gene was inserted downstream of Defb13 and a MICER clone carrying exons 3-9 of the HPRT gene, a puro cassette, a loxP site and Agouti gene was inserted upstream of Defb1. Cre mediated recombination removed a 175 kb region encompassing 9 beta defensin genes (Defb1, Defb50, Defb2, Defb10, Defb9, Defb11, Defb15, Defb35 and Defb13) and reconstituting an intact Hprt gene. RT-PCR analysis confirmed the absence of expression of the nine deleted genes in homozygous mice. (J:204187)

Generation of the Del(8Defb1-Defb13)1Jrdo deletion

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(8Defb1-Defb13)1Jrdo Mutation: 0 strains or lines available

References

• Original: J:204187 Zhou YS, et al., Partial deletion of chromosome 8 beta-defensin cluster confers sperm dysfunction and infertility in male mice. PLoS Genet. 2013 Oct;9(10):e1003826
• All: 1 reference(s)