Tg(Acan-cre/ERT2,-luc)1Gbg
Transgene Detail

Summary

• Symbol: Tg(Acan-cre/ERT2,-luc)1Gbg
• Name: transgene insertion 1, George Bou-Gharios
• MGI ID: MGI:5563229
• Synonyms: Acan-CreER-ires-Luc
• Transgene: Tg(Acan-cre/ERT2,-luc)1Gbg Location: unknown
• Alliance: Tg(Acan-cre/ERT2,-luc)1Gbg page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Acan-cre/ERT2,-luc)1Gbg expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Acan

• Mutation details: A construct was generated where four tandem repeats of the A1 enhancer of the aggrecan gene (-9,477 bp to -9,118 bp of Acan) were cloned upstream of the Col2a1 proximal promoter fragment. The cre/ERT2 sequence, followed by an IRES site and the firefly luciferase cDNA, were inserted after these regulatory elements. The construct was validated by transfection into MEFs isolated from Rosa-lacZ reporter mice, and then used to produce transgenic mice. Founders were crossed with reporter-expressing mice and three showed cre-mediated reporter activity. One line was used for further experiments. (J:208367)

Generation of Tg(Acan-cre/ERT2,-luc)1Gbg.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Acan (species not specified) Summary of all recombinase alleles driven by Acan. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:208367 Lo Cascio L, et al., Generation of a mouse line harboring a Bi-transgene expressing luciferase and tamoxifen-activatable creER(T2) recombinase in cartilage. Genesis. 2014 Feb;52(2):110-9
• All: 2 reference(s)