Pds5b^tm1.2Alos
Targeted Allele Detail

Summary

• Symbol: Pds5b^tm1.2Alos
• Name: PDS5 cohesin associated factor B; targeted mutation 1.2, Ana Losada
• MGI ID: MGI:5563482
• Gene: Pds5b Location: Chr5:150597204-150734155 bp, + strand Genetic Position: Chr5, 89.61 cM
• Alliance: Pds5b^tm1.2Alos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205601
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele contains two loxP sites flanking exons 4 and 5 and an FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 4 and 5 floxed. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:205601)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pds5b Mutation: 100 strains or lines available

References

• Original: J:205601 Carretero M, et al., Pds5B is required for cohesion establishment and Aurora B accumulation at centromeres. EMBO J. 2013 Nov 13;32(22):2938-49
• All: 2 reference(s)