Tg(Camk2a-cre/folA)1Amax
Transgene Detail

Summary

• Symbol: Tg(Camk2a-cre/folA)1Amax
• Name: transgene insertion 1, Anton Maximov
• MGI ID: MGI:5563562
• Synonyms: DD-cre
• Transgene: Tg(Camk2a-cre/folA)1Amax Location: unknown
• Alliance: Tg(Camk2a-cre/folA)1Amax page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: trimethoprim
• Mutation: Insertion
• Tg(Camk2a-cre/folA)1Amax expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Camk2a

• Mutation details: A cDNA sequence encoding a mutant destabilizing form (DD) of bacterial dihydrofolate reductase (ecDHFR; folA) attached to cre recombinase cDNA was inserted downstream of the Camk2a promoter in a targeting vector. The construct also contained 5' and 3' introns flanking the DD-cre sequence as well as a 3' SV40 polyadenylation signal. The linearized construct was used for pronuclear injection and two founders carrying the transgene were identified by PCR. These were bred to Ai9 reporter mice and treated with trimethoprim (TMP) to stabilize the cre protein. One line showed high levels of recombination with TMP treatment with low levels of constitutive cre activity in cortex except for the dentate gyrus. The other line showed very low leakiness of cre activity but also low TMP-induced recombination and was not used for biochemical or behavioral studies. (J:201125)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Camk2a (species not specified) Summary of all recombinase alleles driven by Camk2a.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:201125 Sando R 3rd, et al., Inducible control of gene expression with destabilized Cre. Nat Methods. 2013 Nov;10(11):1085-8
• All: 1 reference(s)