Mylk^tm1.1Bph
Targeted Allele Detail

Summary

• Symbol: Mylk^tm1.1Bph
• Name: myosin, light polypeptide kinase; targeted mutation 1.1, B Paul Herring
• MGI ID: MGI:5563563
• Synonyms: CArG^f, MLCK^f
• Gene: Mylk Location: Chr16:34565580-34822790 bp, + strand Genetic Position: Chr16, 24.49 cM
• Alliance: Mylk^tm1.1Bph page

Shorter small intestines in mgi:5563563/mgi:5563563 mgi:2653286/0 and mgi:5563563/mgi:5563564 mgi:2653286/0 mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204964
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: A loxP site was inserted upstream of the CArG (CC(A/T)6GG) regulatory element in exon 15. An FRT- and loxP-flanked neomycin resistance cassette was inserted downstream of the CArG element. Flp-mediated recombination removed the selection cassette and left the CArG element floxed. This allele targets the smMLCK isoform without affecting full-length Mylk or telokin. Western blot analysis confirmed normal expression of smMLCK isoform. (J:204964)

Generation of the Mylk^tm1.1Bph and Mylk^tm1.2Bph alleles

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mylk Mutation: 103 strains or lines available

References

• Original: J:204964 Chen M, et al., Regulation of 130-kDa smooth muscle myosin light chain kinase expression by an intronic CArG element. J Biol Chem. 2013 Nov 29;288(48):34647-57
• All: 1 reference(s)