Crb3^tm1.1Bmar
Targeted Allele Detail

Summary

• Symbol: Crb3^tm1.1Bmar
• Name: crumbs family member 3; targeted mutation 1.1, Ben Margolis
• MGI ID: MGI:5563834
• Synonyms: Crb3^flox
• Gene: Crb3 Location: Chr17:57368583-57372920 bp, + strand Genetic Position: Chr17, 29.65 cM
• Alliance: Crb3^tm1.1Bmar page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206750
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: An FRT-flanked neomycin resistance cassette was inserted downstream of exon 2. Exon 3 was replaced with a floxed exon 3-GFP fusion. Flp-mediated recombination removed the selection cassette. Western blot analysis confirmed extremely low expression levels of the fusion product in lung tissue from heterozygous mice. (J:206750)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crb3 Mutation: 10 strains or lines available

References

• Original: J:206750 Whiteman EL, et al., Crumbs3 is essential for proper epithelial development and viability. Mol Cell Biol. 2014 Jan;34(1):43-56
• All: 1 reference(s)