Psmb9/Psmb8^tm1Klr
Targeted Allele Detail

Summary

• Symbol: Psmb9/Psmb8^tm1Klr
• Name: proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7); targeted mutation 1, Kenneth L Rock
• MGI ID: MGI:5563849
• Gene: Psmb8 Location: Chr17:34417169-34420428 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: Psmb9/Psmb8^tm1Klr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:180765
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Hypomorph, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single targeting event replaced most of exon 1 of Psmb9 with an in frame alkaline phosphatase floxed neo construct and exons 1 - 5 of Psmb8 with a lacZ poly A FRT construct. Immunoblot analysis confirmed the lack of expression of both PSMB9 and PSMB8 in lysates or proteasome preparations from spleens of homozygous mice. In addition expression of Tap1, located between the 2 targeted genes, mRNA and protein were reduced by about 50% in homozygous mice. (J:180765)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Psmb8 Mutation: 15 strains or lines available

References

• Original: J:180765 Kincaid EZ, et al., Mice completely lacking immunoproteasomes show major changes in antigen presentation. Nat Immunol. 2012 Feb;13(2):129-35
• All: 3 reference(s)