Macroh2a2^tm1.1Peh
Targeted Allele Detail

Summary

• Symbol: Macroh2a2^tm1.1Peh
• Name: macroH2A.2 histone; targeted mutation 1.1, John R Pehrson
• MGI ID: MGI:5565229
• Synonyms: macroH2A2 KO
• Gene: Macroh2a2 Location: Chr10:61574444-61619926 bp, - strand Genetic Position: Chr10, 32.27 cM
• Alliance: Macroh2a2^tm1.1Peh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220524
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector was designed to insert a loxP-flanked PGKneo cassette upstream of exon 2 and loxP site downstream of exon 2. Mutant mice were bred with mice carrying Tg(EIIa-cre)C5379Lmgd to remove exon 2 and the neo cassette. Polymorphisms near the gene demonstrated that the targeted gene is in a genomic region derived from the 129S4/Jae parent of the embryo from which the ES cell line was generated. (J:220524)

Expression

In Mice Carrying this Mutation:

35 assay results 2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Macroh2a2 Mutation: 20 strains or lines available

References

• Original: J:220524 Pehrson JR, et al., Mice without macroH2A histone variants. Mol Cell Biol. 2014 Dec;34(24):4523-33
• All: 3 reference(s)