Cspg4^{tm1.1(cre/ERT2)Fki}
Targeted Allele Detail

Summary

• Symbol: Cspg4^{tm1.1(cre/ERT2)Fki}
• Name: chondroitin sulfate proteoglycan 4; targeted mutation 1.1, Frank Kirchhoff
• MGI ID: MGI:5566862
• Synonyms: NG2-CreERT2, NG2^ct2
• Gene: Cspg4 Location: Chr9:56772388-56807154 bp, + strand Genetic Position: Chr9, 30.32 cM
• Alliance: Cspg4^{tm1.1(cre/ERT2)Fki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209130
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Cspg4^{tm1.1(cre/ERT2)Fki} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cspg4 (MGI:2153093)

• Mutation details: A cre/ERT2 open reading frame and Frt-flanked neomycin cassette replaced exon 1 of the Cspg4 (NG2) locus following homologous recombination in E14.1 ES cells. The neo cassette was deleted by crossing mice to flp expressing mice. No Cspg4 protein is detected in brain lysates. (J:209130)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cspg4 (mouse) Summary of all recombinase alleles driven by Cspg4. Alliance mouse Cspg4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cspg4 Mutation: 101 strains or lines available

References

• Original: J:209130 Huang W, et al., Novel NG2-CreERT2 knock-in mice demonstrate heterogeneous differentiation potential of NG2 glia during development. Glia. 2014 Jun;62(6):896-913
• All: 21 reference(s)