Nlgn2^tm1.1Sud
Targeted Allele Detail

Summary

• Symbol: Nlgn2^tm1.1Sud
• Name: neuroligin 2; targeted mutation 1.1, Thomas C Sudhof
• MGI ID: MGI:5567078
• Synonyms: FN2, Nlgn2^cKO
• Gene: Nlgn2 Location: Chr11:69713949-69728610 bp, - strand Genetic Position: Chr11, 42.89 cM
• Alliance: Nlgn2^tm1.1Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229887
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a loxP site followed by a frt-flanked neomycin resistance (neo) cassette upstream of exon 3, and a second loxP site downstream of exon 5. Flp-mediated recombination removed the selection cassette and left exons 3 through 5 floxed. (J:229887)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud	involves: C57BL/6NTac
cn2	Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud Tg(Pcp2-cre)2Mpin/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac
cn3	Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud Nlgn3^tm4.1Sud/Y Tg(Pcp2-cre)2Mpin/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac
cn4	Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud Nlgn3^tm4.1Sud/Y Tg(Pcp2-cre)2Mpin/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac

Phenotypes:

Affected Systems	cn1	cn2	cn3	cn4
show or hide all annotated terms
behavior/neurological	√
behavior/neurological phenotype	N
impaired contextual conditioning behavior	√
impaired cued conditioning behavior	√
decreased anxiety-related response	√
social withdrawal	√
nervous system	√	√	√	√
nervous system phenotype		N	N
abnormal synaptic bouton morphology				√
abnormal synapse morphology	√		√	√
abnormal CNS synaptic transmission	√			√
decreased excitatory postsynaptic current amplitude			√	√
increased excitatory postsynaptic current amplitude		√
abnormal inhibitory postsynaptic currents	√	√		√
abnormal miniature inhibitory postsynaptic currents	√		√	√
decreased miniature inhibitory postsynaptic current amplitude	√		√	√
decreased miniature inhibitory postsynaptic current frequency	√	√	√	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nlgn2 Mutation: 35 strains or lines available

References

• Original: J:229887 Liang J, et al., Conditional neuroligin-2 knockout in adult medial prefrontal cortex links chronic changes in synaptic inhibition to cognitive impairments. Mol Psychiatry. 2015 Jul;20(7):850-9
• All: 16 reference(s)