Hprt1^{tm355(Ple286-icre/ERT2)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm355(Ple286-icre/ERT2)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 355, Elizabeth M Simpson
• MGI ID: MGI:5568226
• Synonyms: mEMS5931, Ple286-icre/ERT2, SOX3-creERT2
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm355(Ple286-icre/ERT2)Ems} page

Mutation origin

• Mutant Cell Line: mEMS5931
• Germline Transmission: Earliest citation of germline transmission: J:204202
• Parent Cell Line: mEMS4855.02 (ES Cell)
• Strain of Origin: B6N.Cg-A^w-J Hprt1^b-m3
• Project Collection: EUCOMMTOOLS

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Hprt1^{tm355(Ple286-icre/ERT2)Ems} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  SOX3 (6658)

  Sox3	(MGI:98365)

• Mutation details: The transgene consists of a RP11-1019.M14 human bacterial artificial chromosome (BAC) retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2 and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector. Next, an iCre/ERT2 fusion gene and an frt-flanked kanamycin cassette were inserted directly at the ATG of the human SOX3 locus. These transgene is targeted as a single copy "knock-in" into the upstream region of the Hprt locus. Transient infection with FLP recombinase removes the kanamycin cassette. (J:270567)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	SOX3 (human) Summary of all recombinase alleles driven by SOX3. Alliance human SOX3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014
• All: 2 reference(s)