Hprt1^{tm364a(CAG-icre/ERT2)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm364a(CAG-icre/ERT2)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 364a, Elizabeth M Simpson
• MGI ID: MGI:5568383
• Synonyms: CAGGS-creERT2, CAGGS-icre/frt/ERT2/frt, Hprt^{tm364a(CAGGS-icre/ERT2)Ems}, mEMS6096
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm364a(CAG-icre/ERT2)Ems} page

Mutation origin

• Mutant Cell Line: mEMS6096
• Germline Transmission: Earliest citation of germline transmission: J:204202
• Parent Cell Line: mEMS4855.04 (ES Cell)
• Strain of Origin: B6N.Cg-A^w-J Hprt1^b-m3
• Project Collection: EUCOMMTOOLS

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Hprt1^{tm364a(CAG-icre/ERT2)Ems} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The transgene was designed with an HS4 insulator region, the 1723 bp ubiquitous CAGGS promoter (CMV early enhancer/chicken beta actin), a chimeric intron cassette, the iCre/ERT2 fusion gene (with F3-frt sites flanking the ERT2 sequences), the mut6-WPRE element, an SV40 early polyA signal, an additional HS4 insulator region, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. (J:270567)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014
• All: 3 reference(s)