Sod2^{tm1(rtTA*M2,tetO)Jsg}
Targeted Allele Detail

Summary

• Symbol: Sod2^{tm1(rtTA*M2,tetO)Jsg}
• Name: superoxide dismutase 2, mitochondrial; targeted mutation 1, Joel Greenberger
• MGI ID: MGI:5568594
• Synonyms: MnSOD^tet
• Gene: Sod2 Location: Chr17:13226726-13237006 bp, + strand Genetic Position: Chr17, 8.75 cM
• Alliance: Sod2^{tm1(rtTA*M2,tetO)Jsg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209713
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Null/knockout)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The MnSOD targeting vector was designed to insert a 5.3 kbp Tet-On regulatory cassette approximately 30 nucleotides upstream of the initiation codon in the first exon. The Tet-On regulatory cassette contains the optimized form of reverse tetracycline controlled transactivator (rtTA-M2), polyA sequence, loxP site, neomycin resistance gene (neo), polyA sequence, loxP site and five copies of tetracycline operator 5' of a minimal cytomegalovirus promoter. The Tet-On regulatory fragment is a modification of the neo version of Tet-Off regulatory cassette. The Tet-Off cassette was converted to Tet-On by changing five codons via site-directed mutagenesis: S12G(GGC), E19G(GGG), A56P(CCC), D148E(GAG) and H179R(CGC). These amino acid changes converted tTA to the M2 form of rtTA (rtTA-M2). (J:209713)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sod2 Mutation: 26 strains or lines available

References

• Original: J:209713 Epperly MW, et al., Conditional radioresistance of Tet-inducible manganese superoxide dismutase bone marrow stromal cell lines. Radiat Res. 2013 Aug;180(2):189-204
• All: 1 reference(s)