Pfn1^{tm2.1(GFP/ACTB)Wit}
Targeted Allele Detail

Summary

• Symbol: Pfn1^{tm2.1(GFP/ACTB)Wit}
• Name: profilin 1; targeted mutation 2.1, Walter Witke
• MGI ID: MGI:5568700
• Synonyms: huGE
• Gene: Pfn1 Location: Chr11:70542676-70545470 bp, - strand Genetic Position: Chr11, 43.21 cM, cytoband A-D
• Alliance: Pfn1^{tm2.1(GFP/ACTB)Wit} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209786
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A GFP cDNA was fused to the 5' end of the human cDNA with a bovine growth hormone polyadenylation sequence. This fusion construct was inserted at the translation initiation site. Cre-mediated recombination removed the floxed neomycin resistance cassette. (J:209786)

Expression

In Mice Carrying this Mutation:

18 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pfn1 Mutation: 12 strains or lines available

References

• Original: J:209786 Gurniak CB, et al., HuGE, a novel GFP-actin-expressing mouse line for studying cytoskeletal dynamics. Eur J Cell Biol. 2007 Jan;86(1):3-12
• All: 3 reference(s)