Nap1l2^tm1Ucr
Targeted Allele Detail

Summary

• Symbol: Nap1l2^tm1Ucr
• Name: nucleosome assembly protein 1-like 2; targeted mutation 1, Ute Christine Rogner
• MGI ID: MGI:5569102
• Synonyms: null mutation of Nap1l2
• Gene: Nap1l2 Location: ChrX:102227782-102230246 bp, - strand Genetic Position: ChrX, 45.97 cM
• Alliance: Nap1l2^tm1Ucr page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:63883
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: 890 bp of coding sequence of the intronless Nap1l2 gene between two HincII restriction endonuclease recognition sites were replaced, in-frame, by a nuclear-localized beta-galactosidase (lacZ) reporter gene and a neomycin resistance gene. The insertion of these cassettes disrupts the reading frame of the remainder of the Nap1l2 gene, so that the resulting fusion protein includes only 5 N-terminal amino acids of the NAP1L2 protein. Beta-galactosidase expression in developing chimeric embryos was first observed by X-gal staining in the tip of the tail at embryonic day (E) 9.5, and by E10.5 throughout the neural tube. By E12.5 the neural tube, ganglia and some upper and lower thoracic muscles were labeled. Histologic brain sections showed strong lacZ expression in the rhombencephalon and spinal cord. (J:63883)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nap1l2 Mutation: 1 strain or line available

References

• Original: J:63883 Rogner UC, et al., Control of neurulation by the nucleosome assembly protein-1-like 2. Nat Genet. 2000 Aug;25(4):431-5
• All: 1 reference(s)