Tas2r131^{tm2.1(cre)Uboe}
Targeted Allele Detail

Summary

• Symbol: Tas2r131^{tm2.1(cre)Uboe}
• Name: taste receptor, type 2, member 131; targeted mutation 2.1, Ulrich Boehm
• MGI ID: MGI:5569285
• Synonyms: Tas2r131^BLiC, Tas2r131^{F/MBLF/M-IRES-Cre}
• Gene: Tas2r131 Location: Chr6:132933875-132934807 bp, - strand Genetic Position: Chr6, 64.03 cM
• Alliance: Tas2r131^{tm2.1(cre)Uboe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200616
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Epitope tag, Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion
• Tas2r131^{tm2.1(cre)Uboe} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tas2r131 (MGI:2681280)

• Mutation details: The Tas2r131 coding region was replaced with an expression cassette encoding the barley lectin (BL) sequence linked through an internal ribosome entry cassette to the cre recombinase sequence. Both flag and c-myc epitopes (for differential transsynaptic tracing) were inserted into the BL sequence at positions 79 and 597. An AFN (tACE-Flpe/NEOr) neomycin selection cassette, flanked by FRT sites was also inserted. The neomycin selection cassette was removed by crossing to Flpe-deleter. This allele is a Tas2r131 null and produces a bicistronic transcript BL and cre from the Tas2r131 promoter. (J:200616, J:209852, J:309106)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tas2r131 (mouse) Summary of all recombinase alleles driven by Tas2r131. Comparative matrix view of recombinase activities Alliance mouse Tas2r131 gene page

Expression

In Mice Carrying this Mutation:

83 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tas2r131 Mutation: 20 strains or lines available

References

• Original: J:200616 Foster SR, et al., Expression, regulation and putative nutrient-sensing function of taste GPCRs in the heart. PLoS One. 2013;8(5):e64579
• All: 5 reference(s)